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Jaffrey Lab

Weill Cornell Medicine
Cornell University
New York, NY
Molecular Biology / Chemical Biology / Bioinformatics

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Jaffrey Lab

  • People
  • Research
  • Publications
  • Reagent requests
  • Positions Available
  • Intranet
  • Useful links
  • Fun stuff
  • Contact us

1.  GX41 antibody for ubiquitin proteomics (Xu, G., Paige, J.S., Jaffrey, S.R. Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling, Nature Biotechnology, 2010) 

This antibody recognizes "ubiquitin remnants," i.e., a diglycyl-lysine, and allows for immunopurification of peptides derived from the ubiquitinated portion of a protein.  Currently this antibody is available from Lucerna Technologies:  http://www.lucernatechnologies.com/

 

2.  Spinach and Broccoli reagents (Paige, J.S., Wu, K.Y., Jaffrey, S.R. RNA mimics of green fluorescent protein, Science, 333:642-646, 2011; and Paige, J.S., Nguyen-Duc, T., Song, W., Jaffrey, S.R. Fluorescence imaging of cellular metabolites with RNA, Science, 335:1194, 2012)

The fluorescent dyes DFHBI and DFHBI-1T are available from Lucerna Technologies..  The plasmids are available from Addgene: https://www.addgene.org/Samie_Jaffrey/  

 

3. 2-Ethynyl adenosine for poly(A) tail synthesis mapping (Curanovic, D., Cohen, M., Slagle, C.E., Singh, I., Leslie, C.S., Jaffrey, S.R. Transcriptome-wide profiling of stimulus-induced polyadenylation in living cells using a poly(A) trap. Nature Chemical Biology, 9:671-3, 2013).  Ethynyl adenosine can be purchased from Jena Bioscience (catalog number CLK-N005-1).  Click here for this compound.

 

4. MICAL plasmids (Lundquist, M.R., Storaska, A., Liu, T.-C., Larsen, S.D., Evans, T., Neubig, R.R., Jaffrey, S.R. Redox modification of nuclear actin by MICAL-2 regulates SRF signaling. Cell, 156:563-576, 2014).  Plasmids are available at Addgene.