1. GX41 antibody for ubiquitin proteomics (Xu, G., Paige, J.S., Jaffrey, S.R. Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling, Nature Biotechnology, 2010)
This antibody recognizes "ubiquitin remnants," i.e., a diglycyl-lysine, and allows for immunopurification of peptides derived from the ubiquitinated portion of a protein. Currently this antibody is available from Lucerna Technologies: http://www.lucernatechnologies.com/
2. Spinach and Broccoli reagents (Paige, J.S., Wu, K.Y., Jaffrey, S.R. RNA mimics of green fluorescent protein, Science, 333:642-646, 2011; and Paige, J.S., Nguyen-Duc, T., Song, W., Jaffrey, S.R. Fluorescence imaging of cellular metabolites with RNA, Science, 335:1194, 2012)
The fluorescent dyes DFHBI and DFHBI-1T are available from Lucerna Technologies
The plasmids are available from Addgene: https://www.addgene.org/Samie_Jaffrey/
for more information on the plasmids and their use, and here for maps.
3. 2-Ethynyl adenosine
for poly(A) tail synthesis mapping (Curanovic, D., Cohen, M., Slagle, C.E., Singh, I., Leslie, C.S., Jaffrey, S.R. Transcriptome-wide profiling of stimulus-induced polyadenylation in living cells using a poly(A) trap. Nature Chemical Biology, 9:671-3, 2013). Ethynyl adenosine can be purchased from Jena Bioscience (catalog number CLK-N005-1). Click here
for this compound.
4. MICAL plasmids
(Lundquist, M.R., Storaska, A., Liu, T.-C., Larsen, S.D., Evans, T., Neubig, R.R., Jaffrey, S.R. Redox modification of nuclear actin by MICAL-2 regulates SRF signaling. Cell, 156:563-576, 2014). Plasmids are available at Addgene